blog about Biosellal Launches PCR Kit to Combat African and Classical Swine Fever

As African Swine Fever (ASFV) and Classical Swine Fever (CSFV) continue to threaten global pork production, researchers and farmers face unprecedented challenges in disease detection. The Biosellal Bio-T Kit® CSFV & ASFV PCR test kit emerges as a potential game-changer, offering rapid and accurate diagnosis of these devastating diseases.

Precision Technology Replaces Traditional Methods

Traditional diagnostic approaches often prove time-consuming and unreliable. The new ready-to-use PCR kit features an integrated Internal Positive Control (IPC) that simultaneously detects both CSFV (PPC) and ASFV (PPA) in a single real-time PCR reaction. This eliminates complex preprocessing steps while ensuring sample integrity and reaction efficiency.

French-Developed Quality Standards

Developed under strict French U47-600-2 (AFNOR) standards, the triple swine fever PCR kit reflects Biosellal's commitment to veterinary diagnostic excellence. The company's ISO 9001:2015 certification validates its comprehensive quality management system spanning research, production, and technical support.

User-Friendly Design Enhances Workflow

The kit's distinctive blue Master Mix enables quick identification and rapid thawing, significantly reducing preparation time. Additional quality controls include an External Positive Control (EPC) and negative control (water), while the exogenous IPC boosts sensitivity and specificity across complex sample matrices.

Versatile Sample Compatibility

The diagnostic solution accommodates diverse biological samples including:

• Whole blood (EDTA tubes)
• Serum and plasma
• Cell culture supernatants
• Organ tissues (spleen, tonsils, lymph nodes)
• Swabs (blood or exudates)

The system supports single or combined analysis of up to 10 different matrices, making it suitable for both clinical diagnostics and epidemiological studies.

Cross-Platform Compatibility

Validated on AriaMx™ (default fast ramp) systems and confirmed on ABI PRISM® 7500 Fast (standard and fast modes) and Rotor-Gene Q platforms, the kit works with any real-time PCR instrument featuring 6-FAM, VIC, and Cy5 detection channels. This flexibility eliminates the need for costly equipment upgrades.

Comprehensive Diagnostic Solution

The complete system includes ASFV quantitative RNA (cARN-ASFV-001) and CSFV quantitative RNA (cARN-CSFV-001) reference materials for calibration and quality control, ensuring result accuracy and comparability.

Storage and Shelf Life Considerations

For optimal performance:

• Store immediately at ≤ -16°C upon receipt
• RNA pathogen detection kits maintain 12-month stability
• DNA pathogen detection kits remain stable for 24 months

Quality Assurance and Technical Support

All PCR kits undergo development and validation according to NF U47 600-2 standards, guaranteeing sensitivity, specificity, and reliability. ELISA kits similarly comply with NF U47-310 and related standards. The company provides dedicated technical support and customized training programs covering real-time PCR, ELISA, sequencing, and digital PCR technologies.