blog about New PCR Test Kit Detects African Swine Fever Genes Accurately

African Swine Fever (ASF), one of the most devastating animal diseases, continues to pose a significant threat to global livestock industries. A newly developed real-time PCR detection kit that simultaneously tests for three critical genetic markers—VP72, CD2v, and MGF505—represents a major advancement in early and accurate ASF diagnosis.

The African Swine Fever Virus (ASFV) possesses a complex genome with proteins that play crucial roles in viral replication, infection, and immune evasion. The VP72 protein serves as ASFV's primary capsid protein, making its gene detection a cornerstone of traditional diagnostic methods. Meanwhile, the CD2v protein functions as a key immunomodulator, facilitating viral infection of host cells and evasion of immune responses—its detection helps assess viral pathogenicity and transmission potential. The MGF505 gene is closely associated with viral replication and host cell interactions.

By targeting these three genetic markers simultaneously, the new diagnostic kit achieves significantly improved sensitivity and specificity, effectively reducing false positives and negatives while providing more reliable technical support for ASF prevention and control.

Based on fluorescent PCR technology, this kit monitors fluorescence changes during DNA amplification in real time, enabling both qualitative and quantitative detection of target genes. Compared to conventional PCR methods, it offers several advantages:

• High sensitivity: Capable of detecting extremely low viral gene copies (as few as 10 copies per test), enabling early infection identification even during low viral load stages.
• Exceptional specificity: Uses primers and probes specifically designed for VP72, CD2v, and MGF505 genes to accurately distinguish target pathogens from non-target substances, eliminating cross-reactions.
• Rapid results: Delivers diagnostic outcomes within 1-2 hours, crucial for timely outbreak response and containment measures.
• User-friendly design: Simplified protocols minimize operational steps and reduce technical skill requirements while decreasing human error.
• Storage stability: Maintains reliability at room temperature with a 24-month shelf life.
• Automation compatibility: Works seamlessly with automated nucleic acid extraction and PCR platforms for high-throughput testing needs.

The emergence of this diagnostic tool provides critical support for early ASF warning systems, precise diagnosis, and outbreak tracing. Beyond helping farms implement timely isolation measures to reduce economic losses, it enables governments to develop data-driven containment strategies, ultimately safeguarding global livestock industries.

The kit contains all necessary reagents—including enzymes, reaction buffers, and controls—in standardized 25μL reactions compatible with most PCR instruments. Available in 48-test and 96-test configurations, it also offers customization options for specialized laboratory requirements.