Bloggen over Klevervet Launches Enhanced ASFV PCR Kit for Livestock Safety

The global livestock industry faces mounting challenges from African Swine Fever Virus (ASFV), a highly contagious pathogen capable of devastating economic losses during outbreaks. Effective disease monitoring and control depend critically on rapid, accurate diagnostic tools. The KleverVet ASFV PCR test kit represents an innovative in vitro diagnostic solution designed to enhance detection capabilities for swine producers worldwide.

Early Detection as Critical Defense

ASFV's rapid transmission and high pathogenicity make early, precise identification essential for outbreak containment. Utilizing real-time quantitative PCR technology, the KleverVet test kit performs qualitative detection of ASFV DNA in clinical samples from domestic and wild swine, including serum, plasma, and tissue specimens. Engineered for speed, accuracy, and reliability, the system delivers timely diagnostic results to inform crucial containment decisions.

Technical Superiority Through Innovation

The test kit's performance advantages stem from rigorous development and quality control:

• Exceptional specificity: Clinical validation demonstrates 97.5% specificity, effectively distinguishing ASFV from similar pathogens to prevent false positives.
• High sensitivity: With 98.5% sensitivity and a detection threshold of 1,000 genomic copies per milliliter, the kit identifies early-stage infections and low viral loads that elude conventional tests.
• Contamination control: Integrated uracil-DNA glycosylase (UDG) and dUTP systems prevent PCR amplification carryover, ensuring result reliability during high-throughput testing.

Broad Compatibility for Diverse Laboratories

The diagnostic solution supports seamless integration with major real-time PCR platforms, including:

• Agilent Mx 3005P QPCR System
• Corbett Research Rotor-Gene 6000
• QIAGEN Rotor-Gene Q5/6 plex Platform
• Bio-Rad CFX96 Systems

This compatibility allows laboratories to implement ASFV testing without requiring additional capital equipment investments.

Applications in Swine Disease Management

Designed for veterinary diagnostics, the test kit serves livestock operations, veterinary laboratories, and disease control centers conducting ASFV surveillance, outbreak investigations, and confirmatory testing. Early detection enables prompt implementation of quarantine and depopulation measures to limit viral spread and economic damage.

Optimized Storage Conditions

To maintain peak performance, the test kit requires specific storage protocols:

• Long-term preservation: 1-year stability at -24°C to -16°C
• Short-term storage: 14-day viability at +2°C to +8°C

The reagents withstand up to 10 freeze-thaw cycles, providing operational flexibility while maintaining stability during repeated use.